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KMID : 0378019720150050129
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New Medical Journal
1972 Volume.15 No. 5 p.129 ~ p.135
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Effects of Hypophysectomy and of Some Hormones Administered after the Operation on the Mucus Secreting Cells of Rat Colon
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Abstract
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This experiment was worked out to study histochemically the effects of hypophysectomy and some
hormones adminstered after the operation on the mucus secreting cells of the colon of the rat. Healthy male Swiss albino rats weighing about 200 gm were used in this experiment. Hypophysectomy was done by Falconi & Rossi´s transauricular method.
Administered hormones after the operation were STH (2.5 mg/kg, Raben type), ACTH (1.25IU/kg), DOCA (1 mg/kg) and D-thyroxine (0. 05 mg/kg).
Animals of each group were sacrificed on 3rd and 7th day after the treatment. Sham operation group and saline injected group were used as the control.
Specimens from the colons were fixed in 10% neutral formalin solution, embedded in paraffin wax, sectioned in 6u thickness, and stained with periodic acid-Schiff (PAS)-hematoxylin, alcian blue pH 2.5-PAS, and alcian blue pH 1.0-PAS respectively.
Observed results were as follows:
1. Hypophysectomy resulted in distinct decrease of mucosubstance in the mucus secreting cells of
the colon, especially of sulfomucin in histochemical nature and of those in the lower part of intestinal gland in their location. This is provably due to metabolic disturbance of precursors of mucosubstances.
2. Administration of each hormone after the hypophyectomy effectuate the increase of mucous content
of colonic mucosa. These hormones provably not directly act to mucus secreting cells of the
colon, but chiefly activate the synthesis of source materials of mucosubstances.
3. Judging from histochemical observation, sulfomucin was provably initial product among the
various mucosubstances, and also one of mucosubstance which had rapid turnover.
4. Goblet cells in the lower portion of intestinal glands showed active reaction of mucus production
compared with those in the upper portion of glands and the superficial epithelium.
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KEYWORD
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